Most dart frogs seem to live well in pairs, (1.1.0)*, but to view the full range of behavior it is best to look at larger colonies. If the frog's territory is unlimited, or close to it, then a colony with an equal sex ratio is best. The frogs will be able to migrate to where they have the amount of territory they need. Though competition and fighting will occur the frogs should, mostly, do well.
When space is limited then some of the combative behavior, though natural, is often not what we want to have effecting our studying of the animals. There have been a number of reports of some frogs, (D. pumilio and E. tricolor in particular), killing frogs in the act of defending their territory. Also, when space is limited, breeding behavior can be effected.
In my opinion, the population parameters I've presented allow for viewing a large range of behavior while still allowing the animals to thrive and successfully reproduce.
*This designation is as given as; The number of known male frogs. The number of known females . The number of frogs whose sex is unknown, (often meaning juveniles).
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